Cell Counting Kit-8 (CCK-8)
Selleck Cell Counting Kit-8 (CCK-8)细胞活性检测试剂为一瓶即开即用的溶液,该试剂安全,无放射性,性价比高,为客户提供方便、快速、可靠的使用体验。
客户使用Selleck的发表文献578篇
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产品描述
Selleck Cell Counting Kit-8 (CCK-8)是一种氧化还原反应的指示剂,在电子载体1-Methoxy PMS存在的条件下,利用活细胞中的脱氢酶催化四唑盐WST®-8生成甲臜染料(图1),而且甲臜染料的生成量与活细胞的数量成线性关系(图2)。该试剂的灵敏度高于MTT、XTT、MTS或WST-1等其他四唑盐。
Selleck Cell Counting Kit-8 (CCK-8)细胞活性检测试剂为一瓶即开即用的溶液,该试剂安全,无放射性,性价比高,为客户提供方便、快速、可靠的使用体验。
图1: Working mechanisms of Cell Counting Kit-8 (CCK-8)
图2: 使用Selleck Cell Counting Kit-8 (CCK-8)进行细胞计数和OD值的比较。按不同的密度将K562和Hela细胞接种在96孔板的100 μl培养基中(每个样品进行三个重复). 培养16h后,在孔中添加10 μl Cell Counting Kit-8 (CCK-8),并在培养箱按指定时间进行孵育。用培养基作为空白对照,在酶标仪450 nm下检测光吸收值。
图3: 使用Selleck Cell Counting Kit-8 (CCK-8)进行细胞计数和OD值的比较。按不同的密度将HEK293细胞接种在96孔板的100 μl培养基中(每个样品进行三个重复). 培养16h后,在孔中添加10 μl Cell Counting Kit-8 (CCK-8),并在培养箱按指定时间进行孵育。用培养基作为空白对照,在酶标仪450 nm下检测光吸收值。
储存条件(自收到货起)
Selleck Cell Counting Kit-8 (CCK-8)在避光条件下可在室温保存6个月,2-8°C保存2年,-20°C及以下可保存更长时间。