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产品描述
背景知识
内源蛋白的产生和降解一般都维持在一个平衡状态,因此在稳定的环境因素下细胞内的蛋白含量是也是稳定的。但是在体外研究中,蛋白合成过程终止,而降解大大增强,从而影响蛋白得率。因此我们建议使用多种小分子组成的抑制剂混合物来抑制蛋白酶的功能,阻止蛋白降解情况的发生。
产品成分
成分 | 浓度 | 靶点 |
---|---|---|
AEBSF | 104 mM | Serine proteases |
Aprotinin | 80 μM | Serine proteases |
Bestatin | 5 mM | Aminopeptidases |
E-64 | 1.5 mM | Cysteine proteases |
Leupeptin | 2 mM | Serine and cysteine proteases |
Pepstatin A | 1.5 mM | Aspartic proteases |
产品细节
描述: | 6种广谱蛋白酶抑制剂的混合物,保护蛋白完整性 |
---|---|
运输条件: | 蓝冰运输 |
注意点: | 传统的蛋白酶抑制剂(E-64, AEBSF, bestatin, leupeptin and aprotinin)是不能够抑制某些去泛素化(DUB)蛋白酶(例如ATAXIN-3)的功能的。(参考文献: Neil D. Rawlings, Guy Salvesen et al. Handbook of Proteolytic Enzymes,Vol.1, 2012). |
储存条件(自收到货起)
产品应保存于-20°C,有效期2年。使用时请室温放置,用完立即放回-20°C。